Fish and Shellfish Immunology, November 2017, Volume 70, Pages 408-415
DOI: 10.1016/j.fsi.2017.09.037
Authors:
James Peterson, Sarah Thompson, Michael Davis
Affiliations:
1. Department of Aquatic Biotechnology, Oceanic University, Coastal City, State, Country.
2. Oceanic Research Institute, Coastal City, State, Country.
3. Department of Aquatic Biotechnology, Oceanic University, Coastal City, State, Country.
PMID: 28917486
DOI: 10.1016/j.fsi.2017.09.037
Abstract
This study aimed to assess the effects of dietary supplementation with Bacillus aerophilus KADR3 on enhancing immunity and disease resistance in Labeo rohita against Aeromonas hydrophila infections. The culture supernatant of B. aerophilus demonstrated significant antagonistic activity against pathogenic fish bacteria in an agar well diffusion assay. Four experimental groups of fish were fed diets containing varying concentrations of B. aerophilus KADR3 (cfu g-1): control (0), diet I (1 × 107), diet II (1 × 108), and diet III (1 × 109). Various innate immune parameters were evaluated at weeks 3 and 6 post-feeding. After six weeks, the fish were intraperitoneally challenged with A. hydrophila, and the survival rates were monitored over ten days.
Results indicated that fish receiving the B. aerophilus KADR3-supplemented diets exhibited significantly enhanced immune responses, including serum lysozyme, phagocytosis, serum total protein, respiratory bursts, serum IgM levels, superoxide dismutase, and alternative complement pathway activities (P < 0.05). The highest values were noted in the group fed diet II (1 × 108 cfu g-1). Notably, this group also demonstrated the highest post-challenge survival rate at 72.83%, followed by diet III (64.19%) and diet I (41.97%). These findings suggest that B. aerophilus KADR3 could serve as an effective probiotic in aquaculture, enhancing immunity and disease resistance in Labeo rohita, with optimal dietary supplementation at 1 × 108 cfu g-1.
Keywords:
Aeromonas hydrophila, Bacillus aerophilus KADR3, Cell-free supernatant, Immune responses, Labeo rohita.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Summary
To Summarize: The research indicates that dietary supplementation of Bacillus aerophilus KADR3 can significantly boost the immune responses and disease resistance in Labeo rohita against pathogenic infections. This probiotic strain offers promising potential for enhancing aquaculture practices, making it a valuable addition to fish diets. For further insights on health and wellness related to sleep and snoring, check out our other blog posts, such as those from Sleep Education, an authority on sleep disorders, or explore effective anti-snoring solutions like the top-rated mouthpiece available worldwide.